Middlebury
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CHEM0313Y-S14

Biochemistry Laboratory
Biochemistry Laboratory Lab

Biochemistry Laboratory
Experimental biochemistry emphasizing the isolation, purification and characterization of enzymes and the cloning of genes and expression of recombinant protein. Traditional biochemical techniques such as UV-VIS spectroscopy, gel filtration, ion exchange and affinity chromatography, electrophoresis, and immunoblotting will be used in the investigation of several enzymes. Specific experiments will emphasize enzyme purification, enzyme kinetics, and enzyme characterization by biochemical and immunochemical methods. Major techniques in molecular biology will be introduced through an extended experiment that will include DNA purification, polymerase chain reaction, bacterial transformation, DNA sequencing, and the expression, purification, and characterization of the recombinant protein. Class discussions emphasize the underlying principles of the biochemical and molecular techniques employed in the course, and how these experimental tools are improved for particular applications. Laboratory reports stress experimental design, data presentation, and interpretation of results. (CHEM 0322) 2 hr. lect., 6 hrs. lab.
Course Reference Number (CRN):
21273

Course

CHEM0313

All Sections in Spring 2014

Spring 2014

CHEM0313A-S14 Lecture (Sandwick)
CHEM0313Y-S14 Lab (Sandwick)
CHEM0313Z-S14 Lab (Sandwick)