Middlebury
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CHEM0313

Biochemistry Laboratory

Biochemistry Laboratory
Experimental biochemistry emphasizing the isolation, purification and characterization of enzymes and the cloning of genes and expression of recombinant protein. Traditional biochemical techniques such as UV-VIS spectroscopy, gel filtration, ion exchange and affinity chromatography, electrophoresis, and immunoblotting will be used in the investigation of several enzymes. Specific experiments will emphasize enzyme purification, enzyme kinetics, and enzyme characterization by biochemical and immunochemical methods. Major techniques in molecular biology will be introduced through an extended experiment that will include DNA purification, polymerase chain reaction, bacterial transformation, DNA sequencing, and the expression, purification, and characterization of the recombinant protein. Class discussions emphasize the underlying principles of the biochemical and molecular techniques employed in the course, and how these experimental tools are improved for particular applications. Laboratory reports stress experimental design, data presentation, and interpretation of results. (CHEM 0322) 2 hr. lect., 6 hrs. lab.
Subject:
Chemistry and Biochemistry
Department:
Chemistry & Biochemistry
Division:
Natural Sciences
Requirements Fulfilled:
CW
Equivalent Courses:

Sections

Spring 2015

CHEM0313A-S15 Lecture
CHEM0313Y-S15 Lab
CHEM0313Z-S15 Lab

Spring 2014

CHEM0313A-S14 Lecture (Sandwick)
CHEM0313Y-S14 Lab (Sandwick)
CHEM0313Z-S14 Lab (Sandwick)

Spring 2013

CHEM0313A-S13 Lecture (Taurog)
CHEM0313Y-S13 Lab (Taurog)

Spring 2012

CHEM0313A-S12 Lecture (Sandwick)
CHEM0313Y-S12 Lab (Sandwick)

Spring 2011

CHEM0313A-S11 Lecture (Sandwick)
CHEM0313Y-S11 Lab (Sandwick)
CHEM0313Z-S11 Lab (Sandwick)

Spring 2010

CHEM0313A-S10 Lecture (Sandwick)
CHEM0313Y-S10 Lab (Sandwick)
CHEM0313Z-S10 Lab (Sandwick)

Spring 2009

CHEM0313A-S09 Lecture (Moffett)
CHEM0313Z-S09 Lab (Moffett)

Spring 2008

CHEM0313A-S08 Lecture (Daugherty)
CHEM0313Z-S08 Lab (Daugherty)

Spring 2007

CHEM0313A-S07 Lecture (Daugherty)
CHEM0313Z-S07 Lab (Daugherty)

Spring 2006

CHEM0313A-S06 Lecture (Daugherty)
CHEM0313Z-S06 Lab (Daugherty)

Spring 2005

CHEM0313A-S05 Lecture (Sandwick)
CHEM0313Z-S05 Lab (Sandwick)

Spring 2004

CHEM0313A-S04 Lecture (Cluss)
CHEM0313B-S04 Lecture (Cluss)
CHEM0313Z-S04 Lab (Cluss)