CHEM 0313

Biochemistry Laboratory

Biochemistry Laboratory
Experimental biochemistry emphasizing the isolation, purification and characterization of enzymes and the cloning of genes and expression of recombinant protein. Traditional biochemical techniques such as UV-VIS spectroscopy, gel filtration, ion exchange and affinity chromatography, electrophoresis, and immunoblotting will be used in the investigation of several enzymes. Specific experiments will emphasize enzyme purification, enzyme kinetics, and enzyme characterization by biochemical and immunochemical methods. Major techniques in molecular biology will be introduced through an extended experiment that will include DNA purification, polymerase chain reaction, bacterial transformation, DNA sequencing, and the expression, purification, and characterization of the recombinant protein. Class discussions emphasize the underlying principles of the biochemical and molecular techniques employed in the course, and how these experimental tools are improved for particular applications. Laboratory reports stress experimental design, data presentation, and interpretation of results. (CHEM 0322) 2 hr. lect., 6 hrs. lab.
Chemistry and Biochemistry
Chemistry & Biochemistry
Natural Sciences
Requirements Fulfilled:
Equivalent Courses:


Spring 2025

CHEM0313A-S25 Lecture
CHEM0313Z-S25 Lab

Spring 2024

CHEM0313A-S24 Lecture (Dovey)
CHEM0313Z-S24 Lab (Dovey)

Spring 2023

CHEM0313A-S23 Lecture (Dovey)
CHEM0313Z-S23 Lab (Dovey)

Spring 2022

CHEM0313A-S22 Lecture (Dovey, French)
CHEM0313Z-S22 Lab (Dovey, French)

Spring 2021

CHEM0313A-S21 Lecture (Dovey)
CHEM0313Z-S21 Lab (Dovey)

Spring 2020

CHEM0313A-S20 Lecture (Giddings)
CHEM0313Z-S20 Lab (Giddings)

Spring 2019

CHEM0313A-S19 Lecture (Cluss)
CHEM0313Z-S19 Lab (Cluss)

Spring 2018

CHEM0313A-S18 Lecture (Giddings, Cluss)
CHEM0313Z-S18 Lab (Giddings, Cluss)

Spring 2017

CHEM0313A-S17 Lecture (Cluss, Giddings)
CHEM0313Z-S17 Lab (Cluss, Giddings)

Spring 2016

CHEM0313A-S16 Lecture (Cluss, Giddings)
CHEM0313Z-S16 Lab (Cluss, Giddings)

Spring 2015

CHEM0313A-S15 Lecture (Sandwick, Giddings)
CHEM0313Y-S15 Lab (Sandwick, Giddings)

Spring 2014

CHEM0313A-S14 Lecture (Sandwick)
CHEM0313Y-S14 Lab (Sandwick)
CHEM0313Z-S14 Lab (Sandwick)

Spring 2013

CHEM0313A-S13 Lecture (Taurog)
CHEM0313Y-S13 Lab (Taurog)

Spring 2012

CHEM0313A-S12 Lecture (Sandwick)
CHEM0313Y-S12 Lab (Sandwick)

Spring 2011

CHEM0313A-S11 Lecture (Sandwick)
CHEM0313Y-S11 Lab (Sandwick)
CHEM0313Z-S11 Lab (Sandwick)

Spring 2010

CHEM0313A-S10 Lecture (Sandwick)
CHEM0313Y-S10 Lab (Sandwick)
CHEM0313Z-S10 Lab (Sandwick)

Spring 2009

CHEM0313A-S09 Lecture (Moffett)
CHEM0313Z-S09 Lab (Moffett)

Spring 2008

CHEM0313A-S08 Lecture (Daugherty)
CHEM0313Z-S08 Lab (Daugherty)

Spring 2007

CHEM0313A-S07 Lecture (Daugherty)
CHEM0313Z-S07 Lab (Daugherty)

Spring 2006

CHEM0313A-S06 Lecture (Daugherty)
CHEM0313Z-S06 Lab (Daugherty)

Spring 2005

CHEM0313A-S05 Lecture (Sandwick)
CHEM0313Z-S05 Lab (Sandwick)

Spring 2004

CHEM0313A-S04 Lecture (Cluss)
CHEM0313B-S04 Lecture (Cluss)
CHEM0313Z-S04 Lab (Cluss)